Go Back


Creates a multi-record FASTA file containing reference sgRNA sequences. This module is a member of a suite of CRISPR modules designed to assist in the processing of FASTQ-formatted short read data generated in CRISPR screen experiments.  FASTQ files containing trimmed sgRNA read sequences will be aligned to the multi-record FASTA file using a fast short read aligner such as bowtie, and the aligned reads may then be quantified.

Operating System:
Original Author:
Chet Birger;Broad Institute
Uploaded by:
cbirger on January 07, 2015 16:22
Version Comment:


Posted by michael_lohmueller on June 07, 2016 06:01

Using your module there appeared an error named ' ImportError: No module named Bio.Seq'. We were wondering if Biopython should be imported before(Line 3: import Bio). Biopython should be installed on our server correctly.

Submit a Comment